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KMID : 0613820130230010137
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Journal of Life Science
2013 Volume.23 No. 1 p.137 ~ p.142
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Role of LPS-activated Macrophages in the Differentiation of Mesenchymal Stem Cells into Smooth Muscle Cells
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Lee Mi-Jeong
Do Eun-Kyoung
Kim Jae-Ho
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Abstract
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Human adipose-derived mesenchymal stem cells (hMSCs) are highly useful for vascular regeneration of injured or inflamed tissue. Lipopolysaccharide (LPS) is a potent activator of macrophages and stimulates macrophages to release inflammatory cytokines. In the present study, we explored the role of LPS-activated macrophages in the differentiation of hMSCs to smooth muscle cells (SMCs). We demonstrated that conditioned medium from LPS-induced macrophages (LPS CM) stimulates differentiation of hMSCs to SMCs, as evidenced by increased expression of smooth muscle-specific markers, including alpha-smooth muscle actin (PGF2α-SMA), smooth muscle-myosin heavy chain, and calponin. LPS induced the secretion of PGF2α from macrophages, and PGF2α treatment stimulated expression levels of SMC-specific markers in hMSCs. Furthermore, small interfering RNA-mediated silencing of the PGF2α receptor inhibited LPS CM-stimulated PGF2α-SMA expression. These results suggest that LPS-activated macrophages promote differentiation of hMSCs to SMCs through a PGF2α-dependent mechanism.
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KEYWORD
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Mesenchymal stem cells, lipopolysaccharide, macrophages, smooth muscle cells, alpha-smooth muscle actin (α-SMA)
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